Prepared Appearance: Light amber, clear to very slightly opalescent. Solution is light amber, clear to very slightly opalescent. Solution: 2.75% solution, soluble in purified water upon warming. Reaction at 25☌: pH 7.3 ± 0.2 Bacto Tryptic Soy Broth without Dextrose Dehydrated Appearance: Light beige, free-flowing, homogeneous. Reaction of 3.0% Solution at 25☌: pH 7.3 ± 0.2 Difco Tryptic Soy Broth (prepared bottles) Appearance: Light to medium tan yellow, clear to trace hazy. Solution: 3.0% solution, soluble in purified water upon warming. Identity Speci?cations Bacto Tryptic Soy Broth Dehydrated Appearance: Light beige, free-flowing, homogeneous. In the development and testing of media for industrial and clinical applications, per the referenced publications. NOTE: Differences in the Identity Specifications and Cultural Response testing for media offered as both Difco /Bacto and BBL brands may reflect differences 20 Difco™ & BBL™ Manual, 2nd Edition User Quality Control 19 Trypticase Soy Broth supplemented with 5% Fildes Enrichment provides growth factors necessary for the cultivation of fastidi- ous organisms. bovis group of streptococci by the 6.5% NaCl tolerance test. 17,18 Trypticase Soy Broth with 6.5% Sodium Chloride is used to differentiate the enterococcal species from the S. 10-16 Because of its capacity for growth promotion, TSB is also rec- ommended for use as the inoculum broth for disc diffusion and agar dilution antimicrobial susceptibility testing as standardized by the Clinical and Laboratory Standards Institute (CLSI). 8 TSB is recommended for testing bacterial contaminants in cosmetics 9,10 and complies with established standards in the food industry. 1 TSB was chosen by the USDA Animal and Plant Health Inspec- tion Service for detecting viable bacteria in live vaccines. 4-7 This formulation is included in the USP as a medium for use in performing microbial enumeration tests and tests for speci?ed microorganisms when testing nonsterile pharmaceutical products. Summary and Explanation Tryptic (Trypticase) Soy Broth (TSB) is a nutritious medium that will support the growth of a wide variety of microorganisms, including common aerobic, facultative and anaerobic bacteria and fungi. Tryptic (Trypticase) Soy Broth meets United States Pharma- copeia (USP), European Pharmacopoeia (EP) and Japanese Pharmacopoeia (JP) 1-3 performance specifications, where applicable. Tryptic Soy Broth without Dextrose, a low carbohydrate for- mulation of Tryptic Soy Broth, is used for cultivating fastidious and nonfastidious microorganisms. Trypticase Soy Broth with 5% Fildes Enrichment is used for the cultivation of fastidious organisms e.g., Haemophilus in?uenzae. from the Streptococcus bovis group of streptococci. Trypticase Soy Broth with 6.5% Sodium Chloride is used to differentiate Enterococcus spp. Because of its capacity for growth promotion, TSB is also recommended for use as the inoculum broth for disc diffusion and agar dilution antimicrobial susceptibility testing as standardized by the Clinical and Laboratory Standards Institute (CLSI).Difco™ & BBL™ Manual, 2nd Edition Bacto Tryptic Soy Broth/Trypticase Soy Broth (Soybean-Casein Digest Medium) Trypticase Soy Broth with 6.5% Sodium Chloride Trypticase Soy Broth with 5% Fildes Enrichment Bacto Tryptic Soy Broth without Dextrose Intended Use Tryptic (Trypticase) Soy Broth (Soybean-Casein Digest Medium) is a general purpose medium used in qualitative procedures for the cultivation of fastidious and nonfastidious microorganisms from a variety of clinical and nonclinical specimens.TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry.TSB was chosen by the USDA Animal and Plant Health Inspection Service for detecting viable bacteria in live vaccines.This formulation is included in the USP as a medium for use in performing microbial enumeration tests and tests for specified microorganisms when testing nonsterile pharmaceutical products.TSB is a nutritious medium that will support the growth of a wide variety of microorganisms, including common aerobic, facultative and anaerobic bacteria and fungi. Trypticase Soy Broth (Soybean-Casein Digest Medium) is a general purpose medium used in qualitative procedures for the cultivation of fastidious and non-fastidious microorganisms from a variety of clinical and nonclinical specimens.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |